
data, whole-genome detection of SNPs and structural variations, ChIP-seq.
Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred. QIAGEN CLC Genomics RNA-seq and single-cell RNA-seq data analysis 318 views In this 90-minute training, users will learn how to easily analyze RNA-seq and single-cell RNA-seq (scRNA-seq) data using QIAGEN CLC Genomics Workbench software. CLC bio was a bioinformatics software company that developed a software suite subsequently purchased by QIAGEN. Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred. CLC Genomics Workbench ( Qiagen) is a graphical user interface (GUI) based bioinformatics software. Small RNAs were extracted from 200l plasma samples with a miRNeasy Serum/Plasma Kit (Qiagen) in accordance with the manufacturer’s protocol. Koch CM, Chiu SF, Akbarpour M, Bharat A, Ridge KM, Bartom ET, Winter DR (2018) A Beginner’s guide to analysis of RNA sequencing data. The RNA sequencing was performed by BIKEN Biomics services (Osaka, Japan). Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-seq. For researchers who need to analyze data from RNASeq studies, CLC Genomics Workbench and Ingenuity Pathway Analysis provide high-impact visualizations and. Byron SA, Van Keuren-Jensen KR, Engelthaler DM, Carpten JD, Craig DW (2016) Translating RNA sequencing into clinical diagnostics: opportunities and challenges. Analyze your RNA-seq and small RNA (miRNA, lncRNA) data with easy-to-use transcriptomics workflows for differential expression analysis at gene and transcript. Ozsolak F, Milos PM (2011) RNA sequencing: advances, challenges and opportunities. The gene expression analysis was performed at RaNA-seq ( ).
Control sequencing data were obtained from the ENA database ( ). Royce TE, Rozowsky JS, Gerstein MB (2007) Toward a universal microarray: prediction of gene expression through nearest-neighbor probe sequence identification. RNA-seq data were analyzed using the CLC genomics Workbench 20.1 software program (Qiagen).
Okoniewski MJ, Miller CJ (2006) Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations.van Hal NL, Vorst O, van Houwelingen AM, Kok EJ, Peijnenburg A, Aharoni A, van Tunen AJ, Keijer J (2000) The application of DNA microarrays in gene expression analysis.Wang Z, Gerstein M, Snyder M (2009) RNA-seq: a revolutionary tool for transcriptomics.